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Okay friends, meet again with me on this video I will give a tutorial on how to do baseline correction, give labels on the ends of the FDR spectrum and also how to smooth
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spectrum FTR in Origin software. Well, baseline correction is important, why? Usually for semi-quantitative spectrum FTR analysis, yes, because the baseline is usually different, and it can also be used for, for example, semi-quantitative analysis to calculate the area of the peak or to calculate the transmission of each peak. For example, like this, yes, this is the baseline, it's still
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different, yes, it's not the same, well, it's not even, we'll just make it, how do we delete it first? Well, it's like this, we have data x and y for the web number and transmission, then we plot it, select the lens, this is already like this, yes, then we select analysis
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in the analysis menu then select pick and baseline then select pick analyzer then select open dialog well we choose the goal what to choose the substrat baseline yes then select next Well, there is already a baseline, yes, a straight line like this, we select user define so we will select the manual, yes, this is the second derivative method, then this is already auto, this is the
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here the points are already auto, we will use the points manually, yes, we remove the enable auto find, then clear all, then we add manually, now we choose where the points are for correction, the baseline is double-click, just double-click here, double-click, double-click, double-click, double-click, double-click, double-click, double-click, now if you have selected and yes,
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Okay, for example, we choose down then select next, we can see the preview, this is the baseline correction, for example, friends, you can want to be edited, you can choose this, modify or add, we choose modify, this can be adjusted, yes, like this, okay, don't let it enter the line like this, friends, or friends can also add this, yes,
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the point again previous then choose add again the point for example here yes okay next Well this is the preview if it's done you can immediately finish it okay then we close minimize first Well, you will get this data, yes, subtract and subtract from transmittance
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Well, we choose this new data, x2 and y2, then we choose the plot length. Well, this is the result of the baseline correction. Well, this is the baseline, it's the same, the baseline is the same, now this is the baseline, it's more the same. Well, this is the baseline correction result, friends, we change the scale first, scale
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4000 to 400 okay, now this is more straight, the baseline is almost the same
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So for semi-quantitative analysis, the results are better. Next, after we do baseline correction, we will do smoothing the top of FDR data so that the top is more visible. The way is to select the analysis menu, then signal processing, then select smooth open dialog.
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Well, here's the method, the method is to choose this one, the manual one, then how many points do we mention, the more points there are, the more smooth it is, yes, for example, 50, choose preview, now this preview is like this, the result of the smoothing is the smoothing result, we enlarge it, for example, here, it looks like we enlarge it again, for example,
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Well, this is the result of the red smoothing, so the mark will be smoother later, just choose OK, then we minimize again, we get new data again, we minimize, minimize, now here is the new data again, the X4 and Y4 substrates that have been smoothing earlier, we plot it again, plot, land,
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Well, this is the smoothing result, yes, change the data again, scale 4000 to 400, now this is the last one, we will determine the point of the peak, yes, in a romantic way or determine the peak and give the label of the peak data FDR manually, yes, how is it like
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we choose analysis pick and baseline then choose pick analyzer open dialog then choose the goal choose find picks then choose next okay next just choose constant baseline mode then choose next choose next again
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Well, this can be auto, yes, auto-fine, for example, we just click, try to find it, if you want to know the key automatically, yes, choose this, then for example, you want to add the key manually, you can also uncheck it from the auto-fine enable, then select clear all, then add, this is how to add a manual key like this,
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we double click, we double click, we double click on the tops, for example, yes, don't, now the tops have come out, then we select finish, now the data is already, for example, the font is too big, we can set it to 10, then we change the format, the position, we can also change it below, for example,
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or above it can also be a cake, we can set it, double-click position below, now the position is already below, then this is done, friends, then friends can also thicken the spectrum, yes, so that it is clearer, like this, for example, wave number, we tidy it up, yes, this is a superscript, this is a transmitter, we just delete the data, this is
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then we can give the frame, yes, view, then show, then check the frame section, this will add the graphics of the box like this, then we delete the line, we turn on the major and minor ticks like this, so it's like this, friends, this is the result of
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the spectrum data of the FDIL has been corrected by the baseline so that it becomes more even, then the label is given at the top and the smoothing is done at the top of the data so that it is better and easier to analyze. Okay friends, thank you for watching this tutorial video, see you in the next video and see you