CSL Pewarnaan Gram BEM FK UNIBOS

In the preparation of the removal and coloring of the first gram tools and materials, ethanol solution 95%, primer colorant, namely crystal violet and safranin, elodin, alcohol swab, coloring spray, bacteria isolate, tweezers, OCD, spidol, glass object, spirit lamp,

an aqua desk, a slide storage tray, a hand spoon, and a microscope as a observation point. Before coloring the gram, we will wash our hands. Then we take a tissue to clean it and use a hand spoon. Before making the preparation, the glass must be used with the patient's identity.

which contains the patient's name, date of birth, and medical record number. The next step is to clean the glass. There are two ways to clean the glass. First, by using alcohol swab. The alcohol swab is injected on the glass, then on the back of the glass by turning the alcohol swab so that it remains sterile. The second way is by using tissue on the top of the glass.

Also on the bottom of the glass object until it is clean. After that, the glass object is heated so that it remains sterile. After that, we dissolve the NaCl solution on the glass object. After dissolving NaCl solution on the glass object, next is to take the specimen of bacteria from the colony. But before that, the host or cell that we will use is squeezed first until it is really squeezed.

Then cool the OC, open the bacteria specimen, then burn the tongue to keep it sterile. Then take the bacteria specimen, burn the tongue again to keep it sterile, then mix the bacteria on the surface of the object evenly. After that, burn the tongue to keep it sterile,

Then wait for the glass to dry. After the glass dries, fix the glass 2-3 times. Next, we will do the gram coloring. The first step is to place the erasers on the color frame. After that, rinse with the use of purple crystal solution for 1 minute. After 1 minute, remove the purple crystal.

Then, rinse with an aqua dash. Do it from the edge of the glass slide and not directed directly above the preparation. Next, rinse the preparation with a liquid solution. And also do it for 1 minute. After 1 minute, dissolve the aqua dash again.

Next, decolorize using 95% ethanol that is given right above the glass of the preparation for 30 seconds. After 30 seconds, dissolve again with aquadesc solution. After that, rinse the preparation using carbolfusil solution for 1 minute. After 1 minute, dissolve the aquadesc solution again until it is completely clean. After that, strain the preparation

or object glass so that the water in the device flows and waits for the device to dry. After the device is dry, the device is ready to be observed under the microscope. After the device is dry, we place the device on the objective table of the light microscope. Then we observe using magnification 10 times.

What we want to emphasize here is if there is a crystal or precipitate If there is a precipitate, it is better to do a repeat gram poron If there is a precipitate, we do a repeat gram poron Then we make sure that the decoloration is going well That is, the surface is shaped like a negative gram color Then if there is a leucocyte cell, then the coloration will also be shaped like a negative gram

Then we determine the thickness of the waste preparation, namely this can be seen if the cells are stacked or stacked high. Okay, if there are cells, then do the calculation of the eposid cells and epithelial cells, at least 20-40 field of view. Okay, next is to rotate the lens until the area of the object's glass is really free from the lens. Then we test the oil mercy 1 ctss

on top of the preparation after that we observe the preparation using magnification 100 times 20 to 40 field to see the morphology of the bacteria whether it is basil or coconut streptobacillus or streptococcus then we can also see the properties of the bacteria whether the properties are negative or positive

then we look at the observation results based on our observation results, we can find the characteristics of bacteria there are two characteristics of bacteria, the first is positive gram bacteria and the second is negative gram bacteria positive gram bacteria are purple and have a focus shape maybe you are wondering why positive gram bacteria can be purple

Because in the primary color, the violet crystal, it absorbs thick peptidoglycan layers. Maybe you can see the results of the observation of positive gram bacteria here. And there is also the result or example of positive gram bacteria, there is Staphylococcus aureus.

The second is the negative gram bacteria. Negative gram bacteria are red and basil-shaped. Maybe you are wondering why negative gram bacteria can be red.

After the decolourisation process using alcohol, the secondary colour, which is safranin, enters the thin peptidoglycan cell layer. Maybe you can also see the results of the observation of the negative gram bacteria here. And there is also an example of the negative gram bacteria, namely Klebsiella pneumonia.